", Tonmoy Sarkar, Ph.D., I really like this system over traditional Western. Screen for processing-related impurities and host-cell related contaminants. ", Jane Gray, Ph.D., Software has many useful features for data analysis. 2023 ProteinSimple. Jesss fluorescent detection capabilities enable two-color protein detection for multiplexing. Just load your samples and reagents into the microplate . Supplier: ProteinSimple RP001. Just load your samples and reagents into the microplate, and Jess does the rest. All Rights Reserved. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Use Jess's fluorescent capabilities and multiplex your proteins for even higher throughput. With a few clicks, you'll be analyzing immunoassay like standard curves and precisely quantifying your protein. Can I use the same protocol of protein lysate preparation from muscle biopsy for preparing. Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems. Formaldehyde fixation, C Fox, F Johnson, J Whiting and P Roller, Journal of Histochemistry and Cytochemistry, 1985; 33:845-853. -Actin (9) Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates Methods in Molecular Biology October 5, . The chemiluminescent reaction is recorded by a CCD camera in a series of images over time. Reproducible and Quantitative. ", Debbie Hicks, Ph.D., ", Jamie Boisvenue, Multiple with chemluminescence & incandescence canal. Bio-Techne uses cookies to provide you with a great website experience. User Guide for Wes P/N 031-108 Revision 7, July 2018 For research use only. Working with low abundance targets or precious samples? Stellar fluorescence modules enable detection of low abundance proteins and multiplexing of multiple targets, including multi-color immunoassays alongside Stellar Total Protein Staining within the same lane. Senior Research Scientist, MiNA Therapeutics, London, UK, "Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps. FAX 612 656 4400. Jess's fluorescent detection lets you maximize the data you get in one shot. Centre for Tissue Engineering & Regenerative Medicine, University Kebangsaan Malaysia (UKM) Medical Centre, "Jess is a user-friendly, multi-purpose platform that gives me the possibility to perform Western blot analysis on precious samples, as well as quantifying proteins expressed in the cells at the same time. This new feature, called RePlex removes the antibodies from the first round of probing to perform either a second round of probing with new antibodies or total protein detection. Snap! Jess from ProteinSimple, an Bio-Techne brand, automated the protein separation & immunodetection of traditional Western blotting. Why bother stripping and reprobing? ", Mary G. Johlfs, M.S., Lane 1: ladder; Lane 2: phospho-Stat3; Lane 3: Stat 3; Lane 4: phospho-Stat3/Stat3. ProteinSimple has developed Simple Western assays for protein sizing and quantitative immunodetection as an alternative to traditional Western blot analysis. This had previously taken weeks and months using traditional Western blotting methods", Krishna C. Yalla, Ph.D., For samples containing 0.2 1.2 mg/mL of protein, a fluorescent total protein assay can be run on Jess using the Fluorescent Protein Normalization Assay Module (see next section on Fluorescent Total Protein Assays). ProteinSimple RePlex Module for Jess - Fisher Sci All rights reserved. By continuing to use this website you acknowledge this and agree to our cookie policy. Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. Bounce to main content . In Vitro Technologies :: JESS Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Jess gets you down to picogram-level sensitivity with just 3 L of starting material. Pop any bubbles observed in the Separation Matrix wells with a pipette tip. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities. Scan to launch video protocol 2021 ProteinSimple. Minneapolis, MN 55413 Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. 4. ", Miranda Orr, Ph.D., For chemiluminescent detection, samples are first incubated with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. Jess, she's like western blot meets ELISA in one. info@proteinsimple.com proteinsimple.com . Do not use past expiration date. Bio-Techne Brands Which Brands are Currently Available on biotechne.com? Privacy Policy. Jess gives you four ways to analyze proteins. It takes away so much of the pipetting and manipulation error. LOD Comparison of Stellar NIR / IR, Chemiluminescence, and Leading Competitor for Fluorescence Western Blotting using RNase A as the target (Learn more). Jess, she's like western blot meets ELISA in one. Toll-free 800 343 7475 With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process. Jess from ProteinSimple, a Bio-Techne brand, automates and protein separation & immunodetection about traditional Western blotting. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies. Jess gives you an easy way to see if your samples contain a consistent protein loadjust load her in-capillary protein normalization reagent into her assay plate and shell take care of the rest. Which Brands are Currently Available on biotechne.com? We will not share your information for any other purposes. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy. Up to 25 samples can be processed in a single run. Copyright 2023 Bio-Techne. Stellar NIR / IR modules for Jess set the standard for fluorescence Western blotting detection sensitivity. FAX 612 656 4400. Even better, shell quantitate the results for you too. The appropriate control and low variability between plates using Simple Western means we can complete our studies in weeks rather than months. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate. Somebody have experience with the WES proteinsimple system? Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. With a few clicks you'll be analyzing immunoassay-like standard curves and precisely quantifying your protein. <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> <> Voltage is applied to enable separation by molecular weight. Jess gives you four ways to analyze proteins. Senior Research Scientist, TCG Lifesciences, "Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. Utilizing the RePlex module even further increases this throughput as I often probe for 5-6 proteins per capillary in a single run including phosphorylated/total protein pairs in the same lane. Jess automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Best part is the low sample volume that is required. By continuing to use this website you acknowledge this and agree to our cookie policy. With her 13 and 25 sample capillary cartridges you'll get the throughput you need, with minimum hands-on time. In just 3 hours you'll have quantitative, size-based data ready for analysis. USA, TEL 612 379 2956 Meet Jess, your protein analysis problem solver. Head of Research Instrumentation, Cancer Research UK Cambridge Institute, "Some of the proteins we wanted to detect are low in abundance and its really hard to get a good amount of protein for traditional Western blot. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. CST Antibodies Validated for Simple Western | CST Blog Come back to fully analyzed and quantitated results in just 3 hours. Copyright 2023 R&D Systems, Inc. All Rights Reserved. And with in-built protein normalization, you'll have the confidence you need in your results, experiment after experiment. ", Melyssa Bratton, Ph.D., Learn how various capillary electrophoresis techniques can be easily implemented along the development path to support gene therapy development. Jess performs Simple Western size-based assays: chemiluminescence or fluorescence immunoassays, and intra-assay protein normalization. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple | Bio-Techne - IDO Recombinant Polyclonal Antibody (711778) ", Dr. Matteo Ferrari, Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. ", Muhammad Dain Yazid, Ph.D., Open Compass software v6.0 or higher (Jess, Abby, or Wes) or Compass 5.0 (NanoPro 1000, Peggy Sue, Sally Sue) and click on the Assay icon. 3. All assay steps from protein separation, immunoprobing, detection, and analysis are fully automated. Chemiluminescent Total Protein Detection on Simple Western. Hi! Stellar NIR / IR detection modules for Jess set the industry standard for Western blotting fluorescence detection sensitivity. Senior Researcher, EURAC Research, "I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Which Brands are Currently Available on biotechne.com? Director of Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans, "By switching my assays over to Simple Western, I am able to increase my throughput, allowing for full dose responses to be generated in each experiment. By continuing to use this website you acknowledge this and agree to our cookie policy. The Protein Normalization Assay Module is appropriate for samples containing 0.2 1.2 mg/mL of protein and comes with both a separation module and the reagents required to do a total protein assay. The Protein Normalization Assay Module on Jess enables you to run a fluorescent total protein assay in the same capillary on the same sample, without sacrificing your NIR, IR or Chemiluminescent detection channels. Learn more about this offer atSimple Western Antibody Submission Promotion. Meet Jess, your protein analysis problem solver. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, "It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. Protein normalization has never been easier with Jess. and it is better than traditional Western blotting? With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process. Western blotting using #Jess #proteinsimple? | ResearchGate Jess from ProteinSimple, a Bio-Techne brand, automatizes this protein separation & immunodetection from traditional Western dabbing. Using 5 times more concentrated Biotin Labeling Reagent, total protein detection using Simple Western can surpass the sensitivity of SDS-PAGE total protein stains including SYPRO Ruby. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Peggy Sue performs size or charge-based capillary electrophoretic immunoassays with chemiluminescence detection. Postdoctoral Fellow, University of Texas Health Science Center, "We had been trying to study receptor proteins with traditional Westerns but it was frustratingwe just could not get it. Plus, use the Stellar Total Protein assay to normalize your multiplexed . Hold plate firmly on bench and carefully peel off evaporation seal. Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University, "The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. Stellar NIR / IR modules for Jess set the standard for fluorescence Western blotting detection sensitivity. Or detect high and low abundance proteins in the same sample, even when they have identical molecular weights. Jess gives you the qualitative Western blot data you are used to seeing. No product specific FAQs exist for this product, however you may, Average Rating: 5 Maximize your time and sample, and get the information you need in one shot, by multiplexing. Wes has been discontinued as of July 30th, 2021. Processing time of 3 hours for 25 samples to dramatically speed up time to results compared to traditional Western blotting protocols. Bio-Techne uses cookies to provide you with a great website experience. Upon receipt, store it immediately at the temperature recommended below. Remove the assay plate lid. Total protein normalization is increasingly required for western blotting data by academic journals and grant agencies due to the improved reliability of normalized expression values. Go further with high sensitivitybest in class fluorescence detection gets all the information you need in one shot. She separates your protein by size and precisely manages antibody additions, incubations, washes, and even the detection steps. stream Insert a capillary cartridge into the cartridge holder. Her in-capillary protein normalization reagent is an easy way to see if your samples contain a consistent protein load and investigate experimental setup and user errors. Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded. By continuing to use this website you acknowledge this and agree to our cookie policy. 1. ", Mary Howell, Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal. endobj NC1839594. Please note this module can only be used with Jess. 0:00 / 6:29 Introduction ProteinSimple: Transferring your Traditional Western Blot to Wes The Scientist Creative Services Division 804 subscribers Subscribe 1.2K views 4 years ago Wes is. Do traditional assays use too much of your precious samples or require you to pool your samples? Protein analysis comes with many challengeslabor-intensive protocols increase time to result and multiple hands-on steps increase user error and data variability. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - ProteinSimple - MedicalExpo Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.

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